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[目的]研究不同温度对黄芩种子发芽特性和次生代谢的调节。[方法]超氧化物岐化酶(SOD)活性测定采用核黄素-NBT法,过氧化物酶(POD)活性测定采用愈创木酚比色法,过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、苯丙氨酸解氨酶和肉桂酸-4-羟化酶活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。[结果]黄芩种子的发芽率、发芽势和发芽指数均受温度影响。黄芩种子的最适发芽温度为20-25℃;与适温相比,在低温和高温下SOD,POD和CAT的活性明显降低,PAL和C4H也呈现相类似的变化趋势,这与其次生代谢物质的变化也相同;次生代谢物质(黄酮类化合物)与其代谢途径中的关键酶(PAL和C4H)具有良好的相关性(r=0.956,r=0.951,P<0.05)。[结论]黄芩种子发芽以及次生代谢物质合成的适宜温度为20℃。黄芩药材中次生代谢物质的含量可通过提高PAL和C4H的活性来提高。
[Objective] The study aimed to study the regulation of germination characteristics and secondary metabolism of Scutellaria baicalensis Georgi under different temperatures. [Method] The activity of superoxide dismutase (SOD) was determined by using riboflavin - NBT method and peroxidase (POD) activity assay using guaiacol colorimetric method, catalase (CAT), ascorbic acid Oxidase (APX), phenylalanine ammonia-lyase and cinnamic acid-4-hydroxylase activity were measured by UV spectrophotometry. Secondary metabolites were determined by high performance liquid chromatography. [Result] The germination rate, germination energy and germination index of Scutellaria baicalensis were affected by temperature. Soybean skullcap seed germination temperature of 20-25 ℃; Compared with the appropriate temperature, at low temperature and high temperature activity of SOD, POD and CAT decreased significantly, PAL and C4H also showed a similar trend, and its secondary metabolism There was also a good correlation between the secondary metabolites (flavonoids) and the key enzymes (PAL and C4H) in their metabolic pathways (r = 0.956, r = 0.951, P <0.05). [Conclusion] The suitable temperature for the seed germination and secondary metabolite synthesis of Scutellariae Radix was 20 ℃. Scutellaria medicinal secondary metabolites in the content can be increased by increasing the activity of PAL and C4H.