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目的:探讨氯胺酮对内毒素休克鼠动脉血浆TNF-α及肺损伤的抑制作用。方法:选择雄性Wister大鼠40只,随机分为五组,经静脉注射内毒素15mg·kg~(-1)(Ⅰ组)或腹腔注射氯胺酮50～200mg·kg~(-1)后再经静脉注射内毒素,然后持续静滴氯胺酮10mg·kg~(-1)·min~(-1)(Ⅱ、Ⅲ、Ⅳ组)。采用酶联免疫法测定TNF-α含量,动脉血气分析测定动脉血氧分压、肺湿重/体重计算肺水含量,并与正常对照组(Ⅴ组)进行比较。结果:与Ⅴ组比较,大鼠注射LPS后Ⅰ组动脉血浆TNF-α浓度和肺水含量较Ⅰ组明显升高,血氧分压明显下降;而注射氯胺酮后动脉血浆TNF-α含量较Ⅰ组明显下降,动脉血氧分压下降和肺水含量增加等肺损伤指标改变有明显改善,并且这种改变与氯胺酮用量有剂量依赖性。结论:氯胺酮可明显改善内毒素引起的动脉血浆TNF-α浓度增加,减轻内毒素介导的急性肺损伤。
Objective: To investigate the inhibitory effect of ketamine on TNF-α and lung injury induced by endotoxic shock in rats. Methods: Forty male Wister rats were randomly divided into five groups. After intravenous injection of endotoxin 15 mg · kg -1 (group Ⅰ) or intraperitoneal injection of ketamine 50 ~ 200 mg · kg -1 After intravenous injection of endotoxin, ketamine (10 mg · kg -1 · min -1) was continuously injected intravenously (group Ⅱ, Ⅲ and Ⅳ). The content of TNF-α was measured by enzyme-linked immunosorbent assay, the partial pressure of arterial oxygen was measured by arterial blood gas analysis, the lung water content was calculated by lung wet weight / body weight, and compared with the normal control group (Ⅴ). Results: Compared with Group V, the levels of TNF-α in arterial plasma and pulmonary water in Group Ⅰ were significantly increased and the partial pressure of blood oxygen decreased significantly in Group Ⅰ compared with GroupⅤ, while the levels of TNF-α in Arterial Plasma after injection of ketamine Group decreased significantly, arterial partial pressure of oxygen and lung water content increased lung injury indicators such as significantly improved, and this change and the dose of ketamine in a dose-dependent manner. CONCLUSION: Ketamine can significantly improve endotoxin-induced increase in plasma TNF-α concentration and attenuate endotoxin-induced acute lung injury.