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目的:研究化学性腰交感神经切除术(CLS)对直结肠慢性刺激性炎症和扩张性伤害性刺激(CRD)的中枢保护作用,为临床医学提供基础研究资料。方法:Wistar大鼠54只,随机分为6组,每组9只,按二因素析因实验设置实验组,另外设立实验对照组和空白对照组。利用直、结肠慢性刺激性炎性致痛模型,切除模型大鼠的腰交感神经后,运用机械扩张刺激观察大鼠行为变化。用Miampamba评分方法改良后疼痛四级评分标准评分,重复3次取均值记录结果。取间脑、脑桥、延髓、颈段(C5～8)、胸段(T3～6)、腰段(L1～3)、骶段(S2～4)脊髓连续切片后,延髓和脊髓用COX2,间脑、脑桥用胶质纤维酸性蛋白(GFAP)免疫组化染色,显微镜下观察相应核团,计数阳性细胞数。结果:LS无论作为预防性手术还是治疗性手术均能使中枢内GFAP和COX2表达阳性的细胞数下降(P<0.05)。NST,C5～8中间带和T3～6中间带外侧核不是直结肠慢性炎症刺激和CRD扩张性伤害刺激上传通路中的中继神经元(P>0.05)。一、二、三、四级反射各组比较直结肠扩张刺激阈值与手术顺序无关而与手术有关(P<0.05)。假手术不影响痛觉阈值(P>0.05),而手术后痛觉阈值明显上调(P<0.05)。结论:化学性腰交感神经切除术(CLS)在直结肠慢性炎症性刺激和CRD伤害刺激过程中能有效的保护中枢神经系统。腰交感?
OBJECTIVE: To study the central protective effect of chemical lumbar sympathectomy (CLS) on the chronic inflammatory irritation and dilative noxious stimulation (CRD) of the colon and to provide basic research data for clinical medicine. Methods: Fifty-four Wistar rats were randomly divided into 6 groups with 9 rats in each group. The experimental group was set up according to the factorial experiment of two factors. In addition, experimental control group and blank control group were set up. The chronic sympathetic inflammatory pain model was used to excise the lumbar sympathetic nerve in rats. The mechanical behavior of rats was observed by mechanical dilatation and stimulation. Using Miampamba score method to improve the score of the four grades of pain score, repeated 3 times to take the average record results. After serial sections of spinal cord of the brain, pons, medulla oblongata, C5 ~ 8, T3 ~ 6, L1 ~ 3 and S2 ~ 4, The brain and pons were stained with glial fibrillary acidic protein (GFAP) immunohistochemically, the corresponding nuclei were observed under the microscope, and the number of positive cells was counted. Results: LS could decrease the number of GFAP and COX2 positive cells in central nervous system (P <0.05) both as a prophylactic and a curative surgery. NST, C5 ~ 8 and T3 ~ 6 medial lateral nucleus were not the relay neurons in the pathways of chronic colon inflammation and CRD dilatation stimulation (P> 0.05). One, two, three and four levels of reflexes in each group compared with the direct colon dilatation and stimulation threshold has nothing to do with the surgical procedure and surgery (P <0.05). Sham surgery did not affect the pain threshold (P> 0.05), but the pain threshold was significantly increased after surgery (P <0.05). Conclusion: Chemical lumbar sympathectomy (CLS) can effectively protect the central nervous system during the chronic inflammatory and CRD injury of the colon. Sympathetic waist?