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目的 :建立血清中盐酸麻黄碱的酸性染料比色测定法。方法 :样品碱化后乙醚萃取物采用酸性染料比色法于波长 411nm处测定血清中盐酸麻黄碱含量。结果 :血清中盐酸麻黄碱线性测定范围为 0 .5～ 15 .0μg.ml- 1 (r=0 .996 1) ,平均回收率为 96 .9% ,RSD为 1.5 % ,日内、日间精密度分别为 3.0 %～ 6 .8%和 4.6 %～ 9.3%。结论 :本法简便、快速、准确 ,可用于盐酸麻黄碱体内定量的有效方法
Objective : To establish an acid dye colorimetric method for the determination of ephedrine hydrochloride in serum. Methods : The sample was alkalized and the content of ephedrine hydrochloride in the serum was determined by the acid dye colorimetric method at a wavelength of 411 nm. Results: The linear determination range of ephedrine hydrochloride in the serum ranged from 0.5 to 15.0 μg.ml-1 (r=0.996 1) with an average recovery rate of 96.9%, RSD of 1.5%, and intraday and intraday precision. The degrees were 3.0% to 6.8% and 4.6% to 9.3%, respectively. Conclusion : This method is simple, rapid and accurate. It can be used for effective quantification of ephedrine hydrochloride in vivo.