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目的探讨不同剂量鱼藤酮损伤大鼠黑质后行为学和酪氨酸羟化酶免疫活性细胞的变化。方法采用大鼠黑质立体定位注射不同剂量鱼藤酮的方法,观察大鼠行为学变化,应用免疫组化染色检测黑质酪氨酸羟化酶(TH)免疫活性细胞的变化。结果给鱼藤酮21d后,2.5μg/μl组大鼠行为测试记分2-4分为主,占91.3%,TH免疫活性细胞百分数为0.22±0.06%;5.0μg/2μl组大鼠行为测试记分4-6分为主,占78.2%,黑质TH免疫活性细胞百分数为0.17±0.03%。结论鱼藤酮可诱导行为学改变和黑质细胞缺失,低剂量表现帕金森病早期的行为学和细胞学特征,较高剂量表现帕金森病晚期的行为学和细胞学特征。
Objective To investigate the changes of behavior and tyrosine hydroxylase immunoreactive cells in substantia nigra of rats exposed to different doses of rotenone. Methods The rat substantia nigra was injected stereotaxially with different doses of rotenone to observe the changes of behavior in rats. Immunohistochemical staining was used to detect the changes of tyrosine hydroxylase (TH) immunocompetent cells. RESULTS: After 21 days of rotenone, behavioral test score of 2.5μg / μl group was 2-4, accounting for 91.3%, the percentage of TH immunocompetent cells was 0.22 ± 0.06%; Score of behavioral test in 5.0μg / 2μl group was 4- 6 divided into the main, accounting for 78.2%, substantia nigra TH immunoreactive cells percentage of 0.17 ± 0.03%. Conclusions Rotenone can induce behavioral changes and lack of substantia nigra cells. Low dose of Parkinson’s disease can be used to show the behavioral and cytological characteristics of early stage of Parkinson’s disease. The higher dose of Parkinson’s disease shows the behavioral and cytological characteristics of late stage of Parkinson’s disease.