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常规提纯银耳伴生菌一香灰菌丝,用试管培养基反复提纯,手续繁多且不易提纯,我们采用玻璃U型管提纯香灰菌丝,不用琼脂培养基,可得到纯的香灰菌丝。其方法是:取1～2厘米口径的玻璃U型管数只,装上木屑培养基,两端用塑料纸包扎严(耐高压塑料纸),灭菌后,按无菌操作,用接种针挑香灰菌丝前端白色处一点,接于U管一端。经培养,菌丝长至另一端后,及时挑取一点放入又一U型管内,这样反复提纯,可获得纯菌丝。
Conventional Purification Tremella fuciformis strains of a gray mycelia, with test tube medium repeated purification, procedures and more difficult to purify, we use glass U-tube Purification of gray mycelium, without agar medium, can be pure gray mycelium. The method is: take 1 ~ 2 cm diameter glass U-tube only, loaded with sawdust medium, both ends wrapped with plastic paper (high pressure plastic paper), sterilized, according to aseptic operation, with the inoculation needle Pick ash mycelium front white a little, then U tube end. After cultivation, the mycelium grows to the other end, promptly picks a little into another U-tube, so repeated purification, obtain pure mycelium.