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目的探讨醋酸甲羟孕酮(MPA)与顺铂(DDP)联用对人卵巢癌耐药细胞CoC1/cDDP体外侵袭能力的影响及其机制。方法CoC1/cDDP细胞分别加入不同浓度的DDP、MPA及MPA+DDP培养,Borden小室检测CoC1/cDDP侵袭能力;流式细胞仪检测G_1期细胞及凋亡细胞,半定量RT-PCR法检测CoC1/cDDP细胞生存素(survivin)-ΔEx3 mRNA及天冬氨酸特异性半胱氨酸蛋白酶(Caspase)-3 mRNA表达。结果经10μg/ml的DDP作用48h,CoC1/cDDP细胞的侵袭能力、凋亡率、survivin-ΔEx3 mRNA及Caspase-3 mRNA表达较对照组均无明显变化(P>0.05);增加DDP浓度至20μg/ml或加用MPA后,细胞侵袭能力明显受到抑制、凋亡率增加、survivin-ΔEx3 mRNA表达下降(P<0.05),而Caspase-3 mRNA表达增加(P<0.01),这些变化与MPA呈剂量效应关系(P<0.05)。结论MPA具有较强的逆转CoC1/cDDP细胞对DDP的耐药作用,CoC1/cDDP的体外侵袭作用受抑制的机理与survivin-ΔEx3 mRNA下调及Caspase -3 mRNA上调有关。
Objective To investigate the effect of medroxyprogesterone acetate (MPA) and cisplatin (DDP) on the invasion of human ovarian cancer cell line CoC1 / cDDP in vitro and its mechanism. METHODS: CoC1 / cDDP cells were cultured in different concentrations of DDP, MPA and MPA + DDP respectively. Borden chamber was used to detect the invasion ability of CoC1 / cDDP. Flow cytometry was used to detect G 1 cells and apoptotic cells. Semiquantitative RT- cDDP cells survivin -ΔEx3 mRNA and caspase-3 mRNA expression. Results After treated with 10μg / ml DDP for 48h, the invasion ability, apoptosis rate, survivin-ΔEx3 mRNA and Caspase-3 mRNA expression in CoC1 / cDDP cells were not significantly different from those in control group (P> 0.05) / ml or MPA, the cell invasion ability was significantly inhibited, the apoptosis rate was increased, the expression of survivin-ΔEx3 mRNA was decreased (P <0.05), while the expression of Caspase-3 mRNA was increased (P <0.01) Dose-effect relationship (P <0.05). Conclusions MPA can reverse the resistance of CoC1 / cDDP cells to DDP. The mechanism of inhibition of CoC1 / cDDP in vitro is related to down-regulation of survivin-ΔEx3 mRNA and up-regulation of Caspase-3 mRNA.