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用Northern杂交和斑点杂交方法分别探测HL-60-AR细胞中七种癌基因的表达及其在诱导分化后的变化。结果表明,在HL-60-AR细胞中c-myc基因高度活跃表达,H-ras基因次之,fos、sis、abl和erb-B基因表达微弱,而K-ras基因表达极微弱或检测不出。当HL-60-AR细胞被药物诱导成熟分化后,c-myc基因表达大为下降,H-ras、fos、sis、abl和erb-B基因表达亦相应降低,而K-ras的表达则无变化。对诱导分化前后c-myc基因拷贝数的比较分析表明,c-myc基因表达下降可能主要发生在基因的转录或/和转录后调节水平。
The expression of seven oncogenes in HL-60-AR cells and their changes after induced differentiation were respectively detected by Northern blot and dot blot hybridization. The results showed that c-myc gene was highly expressed in HL-60-AR cells, followed by H-ras gene, fos, sis, abl and erb-B genes were weakly expressed while K-ras gene expression was very weak or not detected Out When HL-60-AR cells were induced by drug-induced maturation, the expression of c-myc gene was significantly decreased, while the expression of H-ras, fos, sis, abl and erb-B genes was also decreased, while the expression of K- Variety. Comparative analysis of c-myc gene copy number before and after induction of differentiation shows that the decrease of c-myc gene expression may occur mainly in the gene transcription and / or post-transcriptional regulation.