急性髓系白血病H-ras、凋亡及多药耐药的研究

• 作 者: 林凤茹 姚尔固 罗建民 郭晓楠 张学军 任金海 卫俊萍
• 机 构: 河北医科大学第二医院血液病研究所
• 出 处: 河北医科大学学报
• 发表日期: 1999年01期
• 关键词: 白血病 髓细胞/药物疗法 阿糖胞苷/治疗应用 聚合酶链反应/方法 抗药性

目的研究急性髓系白血病（ＡＭＬ）Ｈｒａｓ／Ｐ２１、抗凋亡基因（ｂｃｌ２）、细胞凋亡与多药耐药基因（ｍｄｒ１／Ｐｇｐ）对疗效的影响，并证实流式细胞术（ＦＣＭ）检测Ｐｇｐ和逆转录多聚酶链反应（ＲＴＰＣＲ）检测ｍｄｒ１ｍＲＮＡ间的相关。方法用ＦＣＭ检测８５例初治ＡＭＬ患者的Ｈｒａｓ／Ｐ２１、ｂｃｌ２、细胞凋亡和ｍｄｒ１产物Ｐｇｐ的表达；用ＲＴＰＣＲ检测其中４７例的ｍｄｒ１／ｍＲＮＡ。结果Ｐ２１＋／Ｐｇｐ＋者完全缓解（ＣＲ）率明显低于Ｐ２１－／Ｐｇｐ－者（分别为５８．１％和１００％，Ｐ＜０．０２５），高ｂｃｌ２／Ｐｇｐ＋的ＣＲ率明显低于低ｂｃｌ２／Ｐｇｐ－者（分别为６０％和９６．３％，Ｐ＜０．００５）。Ｐｇｐ＋３１例中２９例ｍｄｒ１ｍＲＮＡ阳性，Ｐｇｐ－１６例中仅２例ｍｄｒ１ｍＲＮＡ阳性。结论Ｐｇｐ＋同时Ｐ２１＋、高ｂｃｌ２者ＣＲ低，ＦＣＭ检测Ｐｇｐ和ＲＴＰＣＲ检测ｍｄｒ１ｍＲＮＡ相关性良好

Objective To investigate the effect of H ras/P21, anti-apoptosis gene (bcl  2), apoptosis and multidrug resistance gene (mdr  1/P  gp) on acute myeloid leukemia (AML) and confirm the efficacy of Flow cytometry (FCM) was used to detect the relationship between Pgp and reverse transcriptase polymerase chain reaction (RTPCR) to detect mdr1 mRNA. Methods The expression of Hras/P21, bcl2, apoptosis and the expression of mdr1 product Pgp in 85 patients with newly diagnosed AML were detected by FCM. 47 cases of mdr1/mRNA were detected by RTPCR. Results The complete remission (CR) rate of P21+/Pgp+ was significantly lower than that of P21–/Pgp– (58.1% and 100%, P<0.025, respectively). High bcl2/Pgp+ The CR rate was significantly lower than those with low bcl-2/P-gp- (60% and 96.3%, respectively, P<0.005). Twenty-nine cases of Pgp+ were positive for mdr 1 mRNA, and only 2 cases of pgp-16 were positive for mdr1 mRNA. Conclusion P  gp + P21 +, high bcl  2 were low CR, FCM detection of P  gp and RT PCR detection of mdr  1 mRNA correlation