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目的 研究Th1和Th2 细胞表面特征性的趋化因子受体CCR5、CCR3在 1型糖尿病发病机制中的作用。方法 对 15例新诊断的 1型糖尿病患者、10例 2型糖尿病患者以及 10例非糖尿病患者分离外周血单个核细胞 (PBMC)在体外培养 ,用流式细胞仪检测CD+4CCR5 +及CD+4CCR3 +淋巴细胞 ,并用酶联免疫吸附技术检测细胞培养上清中细胞因子IL 4、IFN γ的水平。结果 1型糖尿病患者外周血中CD+4CCR5 +淋巴细胞数显著高于 2型糖尿病患者和对照组 ,CD+4CCR3 +显著低于其它两组 (均P <0 .0 5 )。 1型糖尿病患者和 2型糖尿病患者PBMC细胞上清液中的IFN γ水平高于对照组 (均P <0 .0 5 ) ,1型糖尿病患者IL 4水平低于其它两组 (P <0 .0 1)。结论 检测PBMC的CCR5、CCR3表达可以作为反映人类 1型糖尿病免疫活动的标志 ,从而为 1型DM的早期诊断和预防提供线索
Objective To investigate the roles of chemokine receptors CCR5 and CCR3, which are characteristic of Th1 and Th2 cells, on the pathogenesis of type 1 diabetes mellitus. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from 15 newly diagnosed type 1 diabetic patients, 10 type 2 diabetic patients and 10 non-diabetic patients in vitro. Flow cytometry was used to detect CD + 4CCR5 + and CD + 4CCR3 + lymphocytes. The levels of IL-4 and IFN-γ in the cell culture supernatants were detected by enzyme linked immunosorbent assay (ELISA). Results The number of CD + 4CCR5 + lymphocytes in peripheral blood of patients with type 1 diabetes was significantly higher than that of patients with type 2 diabetes mellitus and controls, and the ratio of CD + 4CCR3 + was significantly lower than that of the other two groups (all P <0.05). The level of IFN-γ in PBMC supernatants of type 1 diabetic patients and type 2 diabetic patients was significantly higher than that of the control group (all P <0.05). The level of IL-4 in type 1 diabetic patients was lower than that of the other two groups (P <0. 0 1). Conclusion Detection of CCR5 and CCR3 in PBMCs can be used as a marker of immune activity in human type 1 diabetes so as to provide clues for the early diagnosis and prevention of type 1 DM