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本文通过体外实验探讨了巯基乙酸对大鼠肝匀浆脂质过氧化及葡萄糖-6-磷酸酶活性的影响。巯基乙酸剂量前者为每ml温育液中0.25,1.25和2.50μl;后者为每ml温育液中0.4、2.0和4.Oμl,对照为生理盐水。与肝匀浆在37℃下共同温育后,测定脂质过氧化反应产物丙二醛及葡萄糖-6-磷酸酶活性。结果表明,巯基乙酸在本实验条件下,能导致肝匀浆脂质过氧化并明显抑制葡萄糖-6-磷酸酶活性。
In this paper, we investigated the effect of thioglycolic acid on lipid peroxidation and glucose-6-phosphatase activity in rat liver homogenate in vitro. The former thioglycolic acid dosage was 0.25, 1.25 and 2.50μl per ml of incubation solution; the latter was 0.4, 2.0 and 4.Oμl per ml of incubation solution and the control was saline. After co-incubation with liver homogenate at 37 ° C, the lipid peroxidation products malondialdehyde and glucose-6-phosphatase activity were determined. The results show that thioglycolic acid in the experimental conditions, can lead to liver homogenate lipid peroxidation and significantly inhibited glucose-6-phosphatase activity.