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以肌组织原代培养方法对正常人成肌细胞进行体外培养；用倒置相差显微镜观察成肌细胞的成肌过程，并应用透射和扫描电镜以及免疫细胞化学技术研究成肌细胞的超微结构以及中间丝结蛋白的分布情况。结果显示，体外培养肌细胞可分为缓慢生长、分裂增殖和肌管形成期３个不同的生长、成熟阶段。成肌细胞是幼稚的早期单核肌细胞，体外分裂增殖能力强，可呈克隆生长或（和）簇状分布的不断的成熟分化过程。分裂增殖期成肌细胞胞浆有束状平行排列的肌丝以及线粒体和糖原分布，并呈逐步的分化成熟过程，可相互融合形成肌管，并可与初生肌管进一步融合发育成熟。各期成肌细胞胞浆中间丝结蛋白呈阳性反应，早期生成结蛋白量少且分布不均。上述方法对同期培养的成肌细胞与纤维母细胞可资鉴别。
Normal myoblasts were cultured in vitro with primary cultured muscle tissue. The myoblast myoblast was observed by inverted phase contrast microscope. The ultrastructure of myoblasts was studied by transmission and scanning electron microscopy and immunocytochemistry. Intermediate silk protein distribution. The results showed that in vitro cultured myocytes can be divided into slow growth, proliferation and proliferation of myotubes 3 different stages of growth and maturation. Myoblasts are naïve early mononuclear muscle cells that have strong ability to divide and proliferate in vitro and may exhibit clonal growth or (or) clustered distribution of mature and differentiated processes. Myeloid myofibroblasts in the process of division and proliferation have bundles of myofilaments arranged in parallel and distribution of mitochondria and glycogen, and they undergo gradual process of differentiation and maturation, which can fuse with each other to form myotubes and further fuse with myogenic myoblasts to mature. The myofibroblasts in the cytoplasm of each stage were positive for silk fibroin, with a small amount of hypoalgenin and uneven distribution in early stage. The above method for simultaneous culture of myoblasts and fibroblasts can be identified.