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目的:探讨中国苏南地区汉族人群不稳定型心绞痛(UAP)患者血清白三烯(LT)B4水平与UAP发病风险和花生四烯酸5-脂氧合酶激活蛋白(ALOX5AP)基因SG13S114T/A多态性的相关性。方法:以141例UAP患者(UAP组)和132例非冠心病患者(对照组)为研究对象,应用聚合酶链反应-限制性片段长度多态性方法检测ALOX5AP基因SG13S114T/A多态性,应用酶联免疫吸附试验检测血清中的LTB4水平[以中位数/四分位数间距(M/IQR)表示]。结果:UAP组血清LTB4水平显著高于对照组(352.52/255.48ng/L:200.28/237.10ng/L,P<0.01),经多因素(性别、年龄、高血压、吸烟、糖尿病和血脂)Logistic回归分析显示,血清LTB4水平与UAP的发病风险相关。在UAP组中,ALOX5AP基因SG13S114T/A位点AA、AT和TT三种基因型之间的血清LTB4水平均差异无统计学意义(347.36/201.92ng/L:361.89/262.23ng/L:365.18/268.43ng/L,均P>0.05),且同年龄段亚组(<60岁和≥60岁)的该位点3种基因型之间的血清LTB4水平亦均差异无统计学意义(均P>0.05)。结论:在中国苏南地区汉族人群中,UAP患者血清LTB4水平显著升高,且与UAP的发病风险显著相关,但不受ALOX5AP基因SG13S114T/A多态性的影响。
Objective: To investigate the association between serum leukotriene (B-B4) levels and the risk of developing UAP in patients with unstable angina pectoris (UAP) and the risk of UAP in Chinese Han population of southern Jiangsu Province and the genetic susceptibility of arachidonic acid 5-lipoxygenase activating protein (ALOX5AP) gene SG13S114T / A Correlation of polymorphisms. Methods: The 141 UAP group and 132 non-CHD patients (control group) were enrolled in this study. The polymorphism of SGC13114T / A in ALOX5AP gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Serum levels of LTB4 were measured using enzyme-linked immunosorbent assay [M / IQR]. Results: The level of LTB4 in UAP group was significantly higher than that in control group (352.52 / 255.48ng / L: 200.28 / 237.10ng / L, P <0.01) Regression analysis showed that serum LTB4 level correlated with the risk of UAP. In the UAP group, no significant difference was found in the level of serum LTB4 between AA, AT and TT of ALOX5AP gene SG13S114T / A locus (347.36 / 201.92ng / L: 361.89 / 262.23ng / L: 365.18 / 268.43ng / L, all P> 0.05). There was no significant difference in serum LTB4 levels among the three genotypes in the same age subgroup (<60 and ≥60 years) > 0.05). CONCLUSIONS: In Chinese Han population of southern Jiangsu province, the level of serum LTB4 in patients with UAP is significantly higher than that in UAP patients, but not in the ALOX5AP gene SG13S114T / A polymorphism.