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组织培养再生的植株显示强烈的遗传变异能力,这一发现导致了实现外源基因渐渗的一个新途径。80年代初,W.R.Scowcroft 博士和 P.J.Lankin 博士提出体细胞无性系变异。大麦、燕麦、小黑麦和小麦等物种的体细胞无性系的细胞遗传分析进一步揭示了由于组织培养的结果出现更多的染色体重组(易位、倒置和缺失),这就为利用生物技术提高外源基因渐渗比例从而获得易位系、代换系、附加系提供了科学根据。小麦黄矮病是我国小麦的主要病害之一,至今在普通小麦中还未发现抗性材料。我们的研究旨在探索组织培养诱导易位系把外缘黄矮抗性导入小麦的可能性。
Tissue culture of regenerated plants shows strong genetic variation, a finding that has led to a new way of achieving introgression of exogenous genes. In the early 1980s, Dr. W. R. Scowcroft and Dr. P. J. Lankin proposed somaclonal variations. Cytogenetic analysis of somatic clones of species such as barley, oats, triticale and wheat further revealed that more chromosomal recombination (translocation, inversion and deletion) occurs as a result of tissue culture, which is the result of the use of biotechnology to improve The exogenous gene introgression ratio in order to obtain translocation lines, substitution lines, additional lines provide a scientific basis. Wheat yellow dwarf is one of the main diseases of wheat in our country. So far, no resistant material has been found in common wheat. Our study aimed to explore the possibility that tissue culture-induced translocation lines introduce peripheral yellowish-resistance to wheat.