,Detection of Intracellular Cytokine Production in Peripheral Blood CD3+ T Cells of Patients with Re

• 作 者: QIAN Qifeng(钱起丰) GUO Hongwei(郭红卫)
• 机 构: Shenzhen Institute of Dermatology, Center for STD Control and Research, Shenzhen 518020, China
• 出 处: 中华性传播感染杂志（英文版）
• 发表日期: 2002年1期
• 关键词: Recurrent genital herpes Cytokines Flow cytometry

Objective:To study the role of Th1fTh2 cytokines in the pathogenesis of recurrent genital herpes (RGH), and to better understand the relationship between them.Methods: A two-color immunofluorescent staining of cell surface antigen and intracellular cytokines for flow cytometric analysis was used for CD3, IL-2, IL-10, IL-12,IFN-γ and TNF-α in CD3+ T-lymphocytes in activated peripheral blood mononuclear cells of patients with RGH.Results: Compared to controls, patients with RGH showed fewer CD3+T cells (P＜0.05) and IL-2 producing and IFN-γ producing T cells (P＜0.02 and P＜0.001, respectively)after in vitro stimulation with PMA and ionomycin in the presence of a protein transport inhibitor. More IL-10 producing and IL-12 producing T cells were found in patients with RGH (P＜0.01). There was no significant difference in the number of TNF-α producing cells between RGH patients and controls (P＜0.05).Conclusion: RGH patients showed relatively more Th2 cytokines. The imbalance between Th1 and Th2 cytokines results in inhibitory effects on a series of cell-immune responses, which may play an important role in the pathogenesis of RGH.