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目的研究锰对大鼠体内生殖激素和乳酸脱氢酶(LDH)和多聚ADP核糖聚合酶(PARP)表达的影响,探讨它们在生精细胞凋亡过程中的作用。方法雄性SD大鼠48只,随机分为空白对照组,低剂量组和高剂量组,腹腔注射Mn Cl24周和6周,TUNEL法检测生精细胞凋亡,放射免疫测定血清睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)含量,化学比色测定血清和睾丸LDH含量。免疫组织化学法检测生精细胞PARP表达。结果各染锰组生精细胞凋亡指数、血清FSH、LH及LDH含量升高,血清T、睾丸LDH含量及PARP表达降低。结论锰可使大鼠T和睾丸LDH降低,FSH、LH和血清LDH活性升高,抑制大鼠生精细胞PARP表达,导致生精细胞凋亡。
Objective To study the effect of manganese on the expression of reproductive hormones, lactate dehydrogenase (LDH) and poly ADP ribose polymerase (PARP) in rats and their roles in the apoptosis of spermatogenic cells. Methods 48 male SD rats were randomly divided into blank control group, low dose group and high dose group, intraperitoneal injection of MnCl for 24 weeks and 6 weeks, apoptosis of spermatogenic cells were detected by TUNEL method, serum testosterone (T) Follicle stimulating hormone (FSH), luteinizing hormone (LH) content, serum and testicular LDH levels by chemical colorimetry. Immunohistochemistry was used to detect the expression of PARP in spermatogenic cells. Results The apoptotic index of spermatogenic cells, FSH, LH and LDH contents in serum of various Mn-Mn-treated groups were increased, and the content of LDH and the expression of PARP in serum T, testis were decreased. Conclusions Manganese can reduce the LDH, the FSH, LH and serum LDH activity in rat T and testis, and inhibit the expression of PARP in rat spermatogenic cells, leading to the apoptosis of spermatogenic cells.