中草药注射剂急性毒性安全试验剂量的优选

• 机 构: 广州市药品检验所中草药科药理组,
• 出 处: 新医药通讯
• 发表日期: 1975年01期
• 关键词: 小鼠死亡 试验剂量 急性毒性 一只 简化检验 中草药注射剂 对分法 检样 草药科

内容:动物试验剂量方法:对分法范围:从0.3毫升开始0.3毫升注射一只小鼠死亡,用0.1毫升注射一只小鼠死亡,示检样急性毒性较大活存,用0.2毫升注射一只小鼠死亡,用0.1毫升注射4只小鼠活存,用0.2毫升注射4只小鼠活存,用0.5毫升注射一只小鼠死亡,用0.4毫升注射一只小鼠死亡,用0.3毫升注射4只小鼠活存,用0.4毫升注射4只小鼠活存,用0.5毫升注射4只小鼠效果:简化检验操作,节省时间,较原来方法节省小鼠20～30%。

Content: Animal test dose method: dichotomy range: 0.3 ml from the beginning 0.3 ml injection of one mouse died, one mouse died with 0.1 ml injection, the test sample showed greater acute toxicity to live, with 0.2 ml injection Only mice died, 4 mice survived with 0.1 ml injections, 4 mice survived with 0.2 ml injections, one mouse died with 0.5 ml injections, and one mouse died with 0.4 ml injections, with 0.3 ml 4 mice were injected to survive, 4 mice were injected with 0.4 ml, and 4 mice were injected with 0.5 ml. Effect: Simplify the test operation, save time, and save 20 to 30% of the mice compared with the original method.