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目的:探讨纳他卡林对低氧引起大鼠主动脉内皮细胞损伤的保护作用及其机制。方法:选取大鼠主动脉内皮细胞作为体外低氧损伤的细胞模型,分为正常对照组、低氧模型组、纳他卡林低、中、高剂量组,利用MTT法测定细胞生存率,硝酸还原酶法检测一氧化氮(NO)释放,RT-PCR法检测细胞间粘附因子-1(ICAM-1)、内皮素-1(ET-1)、血管内皮生长因子(VEGF)mRNA水平。结果:纳他卡林三个剂量组均可逆转低氧所致的血管内皮细胞功能改变,包括提高内皮细胞生存活力和NO的释放水平,显著抑制低氧引发的内皮细胞ICAM-1,ET-1,VEGF mRNA表达量的上调。结论:纳他卡林对低氧诱发的血管内皮细胞分泌功能改变、细胞通透性增加及炎性因子的分泌均具有保护作用。
Objective: To investigate the protective effects of natakalim on hypoxia-induced aortic endothelial cell injury and its mechanism. Methods: Rat aortic endothelial cells (HUVECs) were selected as cell models for hypoxia injury in vitro. The cells were divided into normal control group, hypoxia model group and low, middle and high dose natadalin groups. MTT assay was used to determine cell viability, The release of nitric oxide (NO) was measured by reductase method. The levels of ICAM-1, ET-1 and VEGF mRNA were determined by RT-PCR. Results: The three doses of natakalin could reverse the changes of vascular endothelial cell function induced by hypoxia, including increasing the viability of endothelial cells and the level of NO release, and significantly inhibiting the expression of ICAM-1 and ET- 1, VEGF mRNA expression increased. Conclusion: Nattakalim has a protective effect on hypoxia-induced changes in vascular endothelial cell secretion, cell permeability and inflammatory cytokine secretion.