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目的 了解人胃窦粘膜内胃泌素和生长抑素及其mRNA在细胞内的表达和定位。方法 用免疫细胞化学和原位杂交技术。结果 G细胞内的胃泌素主要位于细胞的基部和侧部 ,而其mRNA则位于核周和核上区 ;D细胞内的生长抑素不仅位于细胞的基部 ,也见于细胞突起 ,其mRNA则位于核周、核上区以及突起。G、D细胞均有开放型和闭合型。结论 G细胞为内分泌方式 ,而D细胞在人胃窦部可能存在两种细胞亚群 ,除旁分泌外 ,也有内分泌方式。
Objective To understand the expression and localization of gastrin, somatostatin and their mRNA in human gastric antral mucosa. Methods Immunocytochemistry and in situ hybridization were used. Results Gastrin in G cells mainly located in the basal and lateral parts of the cells, while its mRNA was located in the perinuclear and supranuclear area. The somatostatin in D cells was located not only at the base of the cells, but also in the cells and the mRNA Located in the perinuclear area, nucleus and protrusions. G, D cells are open and closed type. Conclusion G cells are endocrine, and D cells in human gastric antrum may have two cell subpopulations, in addition to paracrine, but also endocrine mode.