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以水稻抗白叶枯病３对近等基因系（ＩＲ２４与ＩＲＢＢ３、ＩＲ２４与ＩＲＢＢ４和ＩＲ２４与ＩＲＢＢ５）为材料，对近等基因系基因组ＤＮＡ进行ＲＡＰＤ分析，结果显示：经１２０个１０－核苷酸随机引物对３对近等基因系基因组ＤＮＡ的ＰＣＲ扩增，有２个引物（ＯＰＫ－１１和ＯＰＮ－０４）在ＩＲＢＢ３中各扩增出１～２条特异性条带；有３个引物（ＯＰＫ－０８、ＯＰＮ－１１和ＯＰＮ－１８）在ＩＲＢＢ４中各扩增出了２条特异性条带；有３个引物（ＯＰＬ－０４、ＯＰＮ－１０和ＯＰＯ－１８）在ＩＲＢＢ５中各扩增出１～２条特异性条带；在轮回亲本中未见有上述类似的片段被扩增，多次重复均获得较一致的结果。因此，推测这些特异性条带（ＤＮＡ片段）分别与水稻抗白叶枯病抗性基因Ｘａ－３、Ｘａ－４和ｘａ－５相关。
Near isogenic lines (IR24 and IRBB3, IR24 and IRBB4 and IR24 and IRBB5) were used as materials in RAPD analysis of rice isolates from rice bacterial blight. The results showed that after 120 10-nucleosides Two primers (OPK-11 and OPN-04) were used to amplify 1 or 2 specific bands in IRBB3 with 3 random primers and 3 pairs of near-isogenic genomic DNA. Three primers (OPK-08, OPN-11 and OPN-18) amplified two specific bands in IRBB4. Three primers (OPL-04, OPN-10 and OPO-18) 1 or 2 specific bands were added; no similar fragment was amplified in the recurrent parent, and more repeated results obtained more consistent results. Therefore, we speculated that these specific bands (DNA fragments) were related to the resistance genes of rice bacterial blight Xa-3, Xa-4 and xa-5, respectively.