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目的:探讨乳腺癌患者CD4+CD25+Foxp3+调节性T细胞(简称Foxp3+Treg)的变化及意义。方法:选择40例乳腺癌患者和32例乳腺良性肿瘤患者,采用流式细胞术检测外周血Foxp3+Treg、CD8+CD28+T细胞、NK细胞水平;用Western blot和RT-PCR病变乳腺组织Foxp3蛋白与m RNA表达。结果:乳腺癌患者外周血中Foxp3+Treg比例较乳腺良性肿瘤患者明显升高,而CD8+CD28+T细胞、NK细胞比例明显降低(均P<0.05),且乳腺癌患者外周血Foxp3+Treg水平与CD8+CD28+T细胞和NK细胞水平呈负相关(r=-0.631,r=-0.578,均P<0.05);乳腺癌患者术后外周血Foxp3+Treg水平较术前明显降低(P<0.05)。乳腺癌组织中Foxp3蛋白与m RNA的表达均较乳腺良性肿瘤组织明显升高(均P<0.05)。结论:Foxp3+Treg和其标记分子Foxp3在乳腺癌患者中的表达增加,且可能通过抑制CD8+CD28+T细胞和NK细胞而产生肿瘤免疫抑制。
Objective: To investigate the changes and significance of CD4 + CD25 + Foxp3 + regulatory T cells (Foxp3 + Treg) in patients with breast cancer. Methods: Forty breast cancer patients and 32 benign breast tumor patients were selected. The levels of Foxp3 + Treg, CD8 + CD28 + T cells and NK cells in peripheral blood were detected by flow cytometry. The expressions of Foxp3 Protein and m RNA expression. Results: The proportion of Foxp3 + Treg in peripheral blood of breast cancer patients was significantly higher than that in benign breast tumors, but the proportion of CD8 + CD28 + T cells and NK cells was significantly lower in breast cancer patients (all P <0.05) (R = -0.631, r = -0.578, all P <0.05). The level of Foxp3 + Treg in postoperative peripheral blood of patients with breast cancer was significantly lower than that before operation (P <0.05), while the levels of CD8 + T cells and NK cells were negatively correlated <0.05). The expression of Foxp3 protein and m RNA in breast cancer tissues were significantly higher than those in benign breast tumors (all P <0.05). Conclusion: The expression of Foxp3 + Treg and its marker Foxp3 in breast cancer patients increased, and tumor immunosuppression may be produced by inhibiting CD8 + CD28 + T cells and NK cells.