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激光散射技术可以快速准确地测定生物大分子、细胞的电泳迁移率,获取分子量、扩散系数、表面电荷等信息;鉴别、监测某些化学反应。这种动态激光散射方法为生物学、医学、化学等学科提供了一种新的研究手段。 我们利用光外差法和改造的电泳装置,包括散射盒、外加方波交变电场等,组装成激光电泳散射仪,研究了不同样品浓度、外电场的强度和周期等因素对迁移率的影响。用多普勒频移测定了频域和时域的电泳迁移率,对散射光线型和造成线型展宽的因素作了分析,记录了功率谱,并用此法初步鉴别了急性淋巴性白血病人血清和正常人血清以及患者和正常人的红细胞。
Laser scattering technology can quickly and accurately determine the electrophoretic mobility of biological macromolecules and cells, and obtain the information of molecular weight, diffusion coefficient and surface charge; identify and monitor certain chemical reactions. This dynamic laser scattering method provides a new research method for biology, medicine and chemistry. We use the optical heterodyne method and the modified electrophoresis device, including the scattering box, plus a square wave alternating electric field, assembled into a laser electrophoresis scatterometer, studied the influence of different sample concentration, the strength and period of the external electric field on the mobility . The frequency shift and time domain electrophoretic mobility were measured by Doppler shift, and the factors of scattered light type and linear broadening were analyzed. The power spectrum was recorded, and the serum of acute lymphoblastic leukemia And normal human serum and erythrocytes in both normal and human subjects.