Treatment with analgesics after mouse sciatic nerve injury does not alter expression of wound healin

• 作 者: Matt C Danzi Dario Motti Donna L Avison John L Bixby Vance P Lemmon
• 机 构: The Miami Project to Cure Paralysis, University of Miami, Lois Pope LIFE Center, Miami, FL, USA“,”De
• 出 处: 中国神经再生研究（英文版）
• 发表日期: 2016年1期
• 关键词: acetaminophen analgesics axon buprenorphine dorsal root ganglia gene expression

Animal models of sciatic nerve injury are commonly used to study neuropathic pain as well as axon regen-eration. Administration of post-surgical analgesics is an important consideration for animal welfare, but the actions of the analgesic must not interfere with the scientiifc goals of the experiment. In this study, we show that treatment with either buprenorphine or acetaminophen following a bilateral sciatic nerve crush surgery does not alter the expression in dorsal root ganglion (DRG) sensory neurons of a panel of genes associated with wound healing. These ifndings indicate that the post-operative use of buprenorphine or acetaminophen at doses commonly suggested by Institutional Animal Care and Use Committees does not change the intrinsic gene expression response of DRG neurons to a sciatic nerve crush injury, for many wound healing-associated genes. Therefore, administration of post-operative analgesics may not confound the results of transcriptomic studies employing this injury model.