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目的 :探讨趋化因子及其受体在强直性脊柱炎 (AS)时的变化及其在关节炎发病机制中的作用。方法 :用含 5 88个基因的cDNA微阵列方法比较 13例AS患者和 7例健康志愿者外周血单个核细胞 (PBMC)基因的表达水平 ;用流式细胞术对PBMC表面C -X -C趋化因子受体 4 (CXCR4 )蛋白表达水平进行检测 ,并以免疫组化方法和ELISA方法检测其配体SDF - 1(基质来源因子 )在滑膜成纤维细胞和滑膜组织的表达情况。结果 :AS的 5 88个基因谱和健康志愿者有明显区别 ,其中趋化因子受体 (CXCR4 )在AS外周血的单核细胞和CD8+ 的T淋巴细胞表达显著高于健康志愿者组 (P <0 . 0 5 )。SDF - 1在AS病人的PBMC、滑液单个核细胞 (SFMC)、关节液成纤维细胞和关节滑膜衬里层细胞的表达也增高。结论 :趋化因子受体CXCR4及其产物在AS患者的PBMC表达明显增多 ,SDF - 1在AS关节炎患者滑膜成纤维细胞和滑膜组织表达增多 ,提示CXCR4及其配体SDF - 1这一信息通道在AS炎症病变的发展与持续中可能起重要作用。
Objective: To investigate the changes of chemokines and their receptors in ankylosing spondylitis (AS) and their roles in the pathogenesis of arthritis. Methods: The cDNA microarray of 5 88 genes was used to compare the expression of peripheral blood mononuclear cells (PBMCs) in 13 AS patients and 7 healthy volunteers. Flow cytometry was used to detect the expression of C -X-C The expression of CXCR4 was detected by immunohistochemistry and ELISA. The expression of SDF - 1 (matrix - derived factor) in synovial fibroblasts and synovial tissues was detected by immunohistochemistry and ELISA. RESULTS: There was a significant difference between the 5 88 genes in AS and healthy volunteers. CXCR4 expression in peripheral blood mononuclear cells and CD8 + T lymphocytes in AS was significantly higher than that in healthy volunteers (P <0. 0 5). The expression of SDF - 1 in PBMC, synovial fluid mononuclear cells (SFMCs), synovial fibroblasts and synovial lining cells of AS patients also increased. CONCLUSION: The expression of CXCR4 and its products in PBMC of AS patients increased significantly. The expression of SDF - 1 in synovial fibroblasts and synovium of AS patients increased, suggesting that CXCR4 and its ligand SDF - 1 A signaling pathway may play an important role in the development and persistence of AS inflammatory lesions.