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目的研究不同生物群解脲支原体对精子质量及精液白细胞的影响。方法使用培养法检测221例男性不育患者及145例可生育人群解脲支原体的感染率,实时荧光定量PCR法鉴定生物群,彩色精子自动分析系统分析精液常规。结果男性不育人群与可生育男性人群的解脲支原体感染率分别为33.6%和24.7%,差异无统计学意义(P>0.05);biovarⅡ群在不育男性感染阳性标本中的检出率为42.7%,显著高于可生育人群阳性标本中的检出率(22.2%),差异有统计学意义(P<0.05);精液常规结果显示,biovarⅡ感染组的精子浓度和白细胞异常增加比例以及biovarⅡ和混合感染组的总活力与未感染组之间差异具有统计学意义(P<0.05)。结论 biovarⅡ感染与精子质量下降和白细胞异常增加密切相关。
Objective To study the effects of Ureaplasma urealyticum (Ureaplasma urealyticum) on sperm quality and sperm white blood cells in different biota. Methods The infection rate of Ureaplasma urealyticum was detected in 221 cases of male infertility and 145 cases of fertile population by culture method. The biota were identified by real-time fluorescence quantitative PCR, and the sperm routine was analyzed by color sperm automatic analysis system. Results The prevalence of Ureaplasma urealyticum was 33.6% and 24.7% in male infertility and fertile men, respectively, with no significant difference (P> 0.05). The positive rate of biovarⅡ in male infertile men was 42.7%, which was significantly higher than that of positive samples (22.2%) in fertile population (P <0.05). The results of sperm routine showed that the sperm concentration and leucocyte anomaly increased proportion of biovarⅡ infection group and biovarⅡ (P <0.05), and there was a significant difference between the total activity and the uninfected group in mixed infection group (P <0.05). Conclusion The infection of biovar Ⅱ is closely related to the decline of sperm quality and the increase of leucocyte abnormalities.