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目的 研究未成熟心肌细胞缺氧 /复氧期间 c- fos,c-myc的表达 .方法 建立 SD大鼠未成熟心肌细胞原代培养及缺氧 /复氧模型 ,应用原位分子杂交技术 ,观察 c- fos,c- myc在心肌细胞核内的表达 .结果 未成熟心肌细胞在缺氧前 c-fos,c- myc m RNA无表达 ,对照 ( )组和 ( )组 c- fos,c- mycm RNA在缺氧 12 0 min开始表达 ,复氧 6 0 min表达程度进一步升高 .牛磺酸保护组 ( 组 )在缺氧 12 0 min时 c- fos,c- mycm RNA为阴性表达 ,在复氧 6 0 min时仅有弱阳性表达 .结论 c- fos,c- myc m RNA表达程度与心肌细胞损伤程度以及心肌细胞内游离钙浓度的变化密切相关 ,c- fos,c- myc基因表达可能对心肌细胞的损伤、修复或增殖起调控作用
Objective To study the expression of c-fos and c-myc during hypoxia / reoxygenation in immature cardiomyocytes.Methods Primary culture and hypoxia / reoxygenation model of SD rat immature cardiomyocytes were established, and in situ hybridization The expression of c-fos and c-myc in the nucleus of cardiomyocytes was detected by immunohistochemical method.Results The expression of c-fos and c-myc m RNA in immature cardiomyocytes was not observed in hypoxic preconditioning group, The expression of RNA was detected at 120 min after hypoxia, and the expression increased at 60 min after reoxygenation.Cytosine protection group (negative) c-fos and c-mycm RNA were negative at 120 min, The expression of c-fos and c-myc m RNA was closely related to the degree of cardiomyocyte injury and the change of intracellular free calcium concentration, and the expression of c-fos and c-myc might be related to the expression of c- Cardiomyocyte injury, repair or proliferation play a regulatory role