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无症状人群大肠癌普查是防治大肠癌的主要手段,能早期发现大肠癌及癌前病变,通过早期治疗,达到治疗、预防目的.普查的方法多采用一、二种试剂进行大便筛查高危人群,然后纤维肠镜检查,但效果并不十分满意.mAb 作为一种敏感性及特异性较高的试剂,被认为有较高的应用价值.我们应用两种 mAb 试剂同时对无症状人群进行粪便筛查,结合纤维肠镜检查,评价两种 mAb 试剂的效果.1 材料和方法1.1 材料受检者1871例全部为无症状广东邮电职工,年龄30岁～70岁,男1297例,女574例,男:女为2:1.1.2 方法所有受检者当天留取新鲜粪便150mg～300mg 于配套采样瓶中送检,用生理盐水5 mL 稀释后作大便金标免疫试验及胶体金试纸条试验,并记录结果.大便金标免疫试剂采用福建三明市蓝波生物公司产品,该试剂应用抗人胃肠癌 mAb 制成,对应抗原为M_r>200kD 的大分子糖蛋白;胶体金试纸条采用北京万华普曼公司产品,该试剂应用抗人血红蛋白制成,对应抗原为人血红蛋白,两种试验只要有一种阳性,作纤维结肠镜检查.用开塞露一个塞肛或电解质洗肠液服后泻至清水,作乙状结肠镜或全结肠镜检查,发现病变取组织作病理检查.对1871例中阴性者随机抽样32例同时进行纤维乙状结肠镜检查.
Asymptomatic population screening for colorectal cancer is the main means to prevent colorectal cancer, early detection of colorectal cancer and precancerous lesions, through early treatment, to achieve the purpose of treatment, prevention. The census method uses one or two reagents for high-risk screening for stool screening , And then the fiber colonoscopy, but the effect is not very satisfactory. mAb as a high sensitivity and specificity of the reagents, is considered to have a higher application value. We use two kinds of mAb reagents at the same time in asymptomatic people feces Screening, combined with fiber colonoscopy, to evaluate the efficacy of the two mAb reagents. 1 Materials and methods 1.1 Materials Subjects were all 1871 were asymptomatic Guangdong Posts and Telecommunications staff, aged 30 to 70 years old, 1297 males, 574 females , Male : Female : 2:1.1.2 Methods All subjects were given 150 mg to 300 mg of fresh feces in the same day to be sampled in matching sampling bottles. After dilution with 5 mL of normal saline, stool gold immunoassay and colloidal gold test strips were diluted. Test and record the results. The stool gold standard immunoreagent was produced by Lanbo Biological Co., Ltd. of Sanming City, Fujian Province. The reagent was made of anti-human gastrointestinal cancer mAb and the corresponding antigen was M_r>200kD macromolecular glycoprotein; colloidal gold test strips. Adopt The products of Beijing Wanhuapuman Co., Ltd. are made of anti-human hemoglobin and the corresponding antigen is human hemoglobin. If there is a positive test in both tests, a colonoscopy examination is performed. The diarrhea was cleared to clear water for sigmoidoscopy or colonoscopy. The lesions were taken for pathological examination. In the 1871 cases, 32 cases were randomly sampled by fiber sigmoidoscopy.