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目的 探讨抑癌基因p1 6在肝癌及癌旁组织中的表达及临床意义。方法 应用免疫组织化学方法对 5 0例肝细胞性肝癌 (HCC)及癌旁肝硬化组织 (44例 )手术切除石蜡包埋标本进行P1 6基因蛋白表达的测定 ,并结合临床病理指标 (Child分级、肿瘤大小、Edmondson分级 ,转移及生存期 )进行分析。结果 p1 6基因在HCC中表达的阳性率为 48% (2 4 5 0 ) ,癌旁 91 % (40 4 4)。p1 6基因表达与HCC的分化程度密切相关 (r =- 0 34 4,P =0 0 1 4 ) ,在分化Ⅰ Ⅱ级中p1 6阳性率为 5 9% (1 9 32 )明显高于Ⅲ Ⅳ级的 2 7 8% (5 1 8)。结论 p1 6基因在HCC的发生及分化中起重要的调控作用 ,且p1 6的失活可能发生在HCC的早期。
Objective To investigate the expression of tumor suppressor gene p1 6 in hepatocellular carcinoma and its adjacent tissues and its clinical significance. Methods Immunohistochemistry was used to detect the expression of P1 6 gene in 50 cases of hepatocellular carcinoma (HCC) and adjacent liver cirrhosis (44 cases). The expression of P1 6 protein was detected by immunohistochemistry and combined with clinicopathological parameters , Tumor size, Edmondson grade, metastasis, and survival) were analyzed. Results The positive rate of p1 6 gene expression in HCC was 48% (2450) and 91% (40 4 4) in adjacent tissues. The expression of p16 gene was closely related to the degree of differentiation of HCC (r = - 0 34 4, P = 0 0 14). The positive rate of p1 6 in differentiation Ⅰ Ⅱ was 59% (1 9 32) Grade IV 278% (5 1 8). Conclusion The p1 6 gene plays an important regulatory role in the occurrence and differentiation of HCC, and the inactivation of p1 6 may occur in the early stage of HCC.