论文部分内容阅读
目的 观察还原型谷胱甘肽 (GSH)对蒿甲醚伍用氯化血红素抗日本血吸虫作用的影响。 方法 将蒿甲醚、氯化血红素、GSH及谷胱甘肽耗竭剂 2 ,4 -二硝基氯苯 (CDNB)单一或伍用加入含 5周龄血吸虫的培养液内 ,温育2 4 h后 ,测定虫体的丙二醛含量 ,并观察培养至 96 h虫体存活情况。感染小鼠经蒿甲醚 30 0 mg/kg治疗后 6、12或 2 4h,测定虫体的 GSH水平。 结果 体外 ,蒿甲醚伍用氯化血红素作用血吸虫 2 4 h后 ,虫丙二醛含量明显升高。培养时间延长 ,虫陆续死亡。GSH对蒿甲醚伍用氯化血红素诱导血吸虫脂质过氧化及杀虫作用具有拮抗作用 ,CDNB则有增强作用。体内 ,蒿甲醚作用血吸虫 6～ 2 4 h,虫体内 GSH水平先降低再明显升高。 结论 GSH可能在血吸虫防御蒿甲醚衍生的毒性过氧化物和自由基攻击中起重要作用。
Objective To observe the effect of reduced glutathione (GSH) on the anti-Schistosoma japonicum effect of artemether and hemin. Methods Artemether, hemin, GSH and glutathione depleting agent 2, 4 - dinitrochlorobenzene (CDNB) were added singly or in combination into the culture medium containing 5 - week - old schistosomiasis and incubated for 24 h, the malondialdehyde content of the parasites was measured, and the survival of the parasites was observed after 96 h culture. The infected mice were treated with artemether 30 0 mg / kg for 6, 12 or 24 hours after treatment to determine the body’s GSH levels. Results In vitro, the worm malondialdehyde content was significantly increased after arthemmether was treated with hemin for 24 h. Training time extended, insects died one after another. GSH antagonized the effect of artemether on the lipid peroxidation and insecticidal action induced by hemin and the enhancement of CDNB. In vivo, the effect of artemether on schistosome 6 ~ 24 h, GSH levels in parasites first and then significantly increased. Conclusion GSH may play an important role in the prevention of artemether-derived toxic peroxides and free radical attack by Schistosoma japonicum.