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目的:观察中药清毒栓对宫颈癌Hela细胞增殖抑制及诱导凋亡的作用。方法:通过MTT比色实验及吖啶橙/溴化乙啶(AO/EB)双染法检测Hela细胞增殖及凋亡的变化。结果:不同剂量清毒栓及保妇康栓药物作用后各组Hela细胞增殖数量明显减少及凋亡率明显增加,其中以作用24 h时对细胞抑制作用最为明显,清毒栓高剂量组分别与正常对照组及清毒栓中、低剂量组比较差异有统计学意义(P<0.05);MTT比色实验中保妇康组与清毒栓高剂量组比较差异有统计学意义(P<0.01),中药清毒栓作用Hela细胞在48 h及72 h时各组之间的比较差异均无统计学意义(P>0.05)。结论:清毒栓对宫颈癌Hela细胞的作用可能是通过抑制肿瘤内皮细胞的增殖诱导凋亡。
Objective: To observe the effect of Qingdu suppository on the proliferation inhibition and apoptosis induction of cervical cancer Hela cells. Methods: MTT colorimetric assay and acridine orange / ethidium bromide (AO / EB) double staining assay Hela cell proliferation and apoptosis. Results: After treated with different doses of Qingdu suppository and Baofukang suppository, the proliferation of Hela cells in each group was significantly decreased and the apoptosis rate was significantly increased. Among them, the inhibition effect on Hela cells was the most obvious at 24 hours, Compared with normal control group and Qingdu Bolus medium and low dose group, the difference was statistically significant (P <0.05); in MTT colorimetric assay, there was significant difference between Baofukang group and Qingduan high dose group (P < 0.01). There was no significant difference in the Hela cells treated with Qingdu suppository at 48 h and 72 h (P> 0.05). Conclusion: The effect of Qingdu suppository on cervical cancer Hela cells may induce apoptosis by inhibiting the proliferation of tumor endothelial cells.