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目的 :探讨RT PCR方法检测腹腔冲洗液中游离细胞的CEAmRNA表达 ,对预测胃癌腹膜亚临床转移的意义。方法 :收集 43例胃癌和 7例胃良性病变患者的术中腹腔冲洗液 ,用RT PCR方法测定冲洗液中游离细胞的CEAmRNA表达 ,同时作冲洗液细胞学检查 (PLC)。结果 :腹腔冲洗液游离细胞的CEAmRNA的表达量随病期的进展、浸润深度的加深而逐渐升高 (P <0 .0 1) ;因胃浆膜类型和大体类型的不同其阳性级别亦存在明显差异 (P<0 .0 1)。结论 :CEART -PCR方法对于检测腹腔微量游离癌细胞较PLC有更高的灵敏度和特异性 ,是一种检测胃癌腹膜微转移的较好方法。
Objective: To investigate the expression of CEA mRNA in free cells in peritoneal lavage fluid by RT PCR method and to predict the significance of subclinical metastasis of gastric cancer. Methods: 43 cases of gastric cancer and 7 cases of benign gastric lesions were collected intraoperatively with intraperitoneal lavage fluid. The free-cell CEA mRNA expression in the rinsing fluid was measured by RT-PCR method and rinse cytology (PLC) was performed at the same time. RESULTS: The expression of CEA mRNA in the peritoneal lavage fluid free cells gradually increased with the progression of the disease stage and the depth of invasion (P < 0.01). The positive levels of CEAmRNA in the peritoneal lavage fluid were also different due to differences in gastric serosal type and gross type. Significant differences (P < 0.01). Conclusion : The CEART-PCR method has higher sensitivity and specificity than the PLC in the detection of peritoneal micro-free cancer cells. It is a good method to detect peritoneal micrometastasis in gastric cancer.