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作者用改进的Franks和Stoll氏法从有微丝蚴血症的斐济人血中浓集和分离班氏丝虫微丝蚴,用Rozeboom和Cabrera的方法从实验感染的蚊体内解剖取出班氏丝虫感染期幼虫。将微丝蚴和感染期幼虫用生理盐水洗一次,在显微镜下用微量毛细摘管吸取后直接放入培养皿(直径为10厘米)中用胶泥环制成的、加满蒸馏水的孔内(孔的直径为5毫米,深度为2毫米),经-20℃冰冻后,在-20℃低温条件下制成8微米薄的冰冻切片,放室温内干燥。在进行萤光抗体试验前,用丙酮处理切片10分钟,然后在37℃再干燥15分钟,贮存于-70℃备用。采用D’Ale-
The authors used a modified Franks and Stoll’s method to concentrate and isolate filamentary filarial filariasis from Fijian blood with microfilaremia, and anatomical removal of Benjamines from experimentally infected mosquitoes using the Rozeboom and Cabrera methods Insect infestation larvae. The microfilariae and infection larvae were washed once with saline, micropipette micropipettes under the microscope and then directly into the Petri dish (diameter of 10 cm) made of clay ring, filled with distilled water hole ( The diameter of the wells is 5 mm and the depth is 2 mm). After frozen at -20 ° C, a thin section of 8 μm thin frozen at -20 ° C is prepared and left to dry at room temperature. Slides were treated with acetone for 10 minutes prior to the Fluorescent antibody test, then dried at 37 ° C for a further 15 minutes and stored at -70 ° C until use. With D’Ale-