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目的：观察Ｉ６５对家兔血小板胞浆游离钙离子（［Ｃａ２＋］ｉ）浓度的影响．方法：用Ｑｕｉｎ２ＡＭ荧光探针在体外测定家兔血小板［Ｃａ２＋］ｉ．结果：ＣａＣｌ２１ｍｍｏｌ·Ｌ－１时，Ｉ６５（１０，２０和３０μｍｏｌ·Ｌ－１）使血小板［Ｃａ２＋］ｉ分别由１４２±２２ｎｍｏｌ·Ｌ－１和１２４±１８ｎｍｏｌ·Ｌ－１减少到１１８±２０，７８±１２，４０±１０ｎｍｏｌ·Ｌ－１和１０８±１５，７７±１４，３７±１４ｎｍｏｌ·Ｌ－１．用依他酸２ｍｍｏｌ·Ｌ－１络合胞外Ｃａ２＋，Ｉ６５胞内贮存Ｃａ２＋释放从５２±１１ｎｍｏｌ·Ｌ－１减少至３４±９，１９±６和１１±５ｎｍｏｌ·Ｌ－１．另外，Ｉ６５也使胞外Ｃａ２＋跨膜内流从９１±１３ｎｍｏｌ·Ｌ－１分别降至８４±１５，５８±１５和２８±１９ｎｍｏｌ·Ｌ－１．结论：Ｉ６５不仅明显抑制家兔血小板胞外Ｃａ２＋跨膜内流而且抑制胞内贮存Ｃａ２＋释放．
Objective: To observe the effect of I65 on the platelet cytoplasmic free calcium ([Ca2 +] i) concentration in rabbits. Methods: Rabbit platelet [Ca2 +] i was measured by Quin2-AM fluorescent probe in vitro. Results: I65 (10, 20 and 30μmol·L-1) decreased platelet [Ca2 +] i from 142 ± 22nmol·L-1 and 124 ± 18nmol·L-1 to 118 ± 20,78 ± 12,40 ± 10 nmol·L-1 and 108 ± 15,77 ± 14,77 ± 14 nmol·L-1. Intracellular Ca2 + was complexed with 2mmol·L-1 of ethacrynic acid, and the intracellular Ca2 + release of I-65 decreased from 52 ± 11nmol·L-1 to 34 ± 9, 19 ± 6 and 11 ± 5nmol·L-1. In addition, I 65 also makes the extracellular Ca2 transmembrane influx decreased from 91 ± 13nmol · L-1 to 84 ± 15,58 ± 15 and 28 ± 19nmol · L-1. Conclusion: I65 not only significantly inhibits the extracellular Ca2 + transmembrane influx in rabbit platelets but also inhibits the intracellular storage of Ca2 + release.