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于1986～1988年,对我区2269份血液标本进行弓形虫抗体检测,发现人群弓形虫感染率为7.0％(103/1471),并进行了人血液弓形虫的分离。 病原分离方法选择具有一定体征、弓形虫抗体阳性人群,取肘静脉抗凝血,进行健康小鼠腹腔接种,每例人血接种3只小鼠,每只0.3ml,从第2代开始,盲传接种液为第一代小鼠实质器官混合研磨液(10％生理盐水),每代7～10d,如此反复盲传至第5
From 1986 to 1988, Toxoplasma gondii antibody was detected in 2269 blood samples in our district, and the population infection rate of Toxoplasma gondii was 7.0% (103/1471), and the isolation of Toxoplasma gondii from human blood was performed. Pathogen isolation method to select a certain signs of Toxoplasma gondii antibody positive population, elbow vein anticoagulant blood, healthy mice were intraperitoneally inoculated, each human blood inoculated 3 mice, each 0.3ml, starting from the second generation, blind Transfer the seed solution to the first generation of mice in real organ mixture grinding solution (10% saline), each generation 7 ~ 10d, so repeatedly blind to 5