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目的:探讨miR-188-5p对肾间质纤维化过程中基质金属蛋白酶(matrixmetalloproteinases,MMPs)表达的影响。方法:采用TGF-β1诱导肾小球系膜细胞(Mesangial cells,MC)纤维化,观察纤维化过程中miR-188-5p和MMP-13的表达变化;通过报告基因实验研究miR-188-5p对MMP-13的调控作用;通过脂质体转染的方法将人工合成的miR-188-5p mimics/inhibitor转入细胞内增加或减少miR-188-5p的表达,进一步观察miR-188-5p对MMP-13表达的影响。结果:TGF-β1诱导肾小球系膜细胞纤维化后miR-188-5p的表达明显升高,而MMP-13表达下调;报告基因实验表明miR-188-5p对MMP-13的表达有明显的调控作用,减少miR-188-5p的表达后MMP-13的表达上调,而增加miR-188-5p的表达后MMP-13的表达下调,呈明显负性调控。结论:在肾间质纤维化过程中,miR-188-5p可能通过抑制MMP-13的表达而发挥促纤维化的作用,本研究为肾间质纤维化的治疗提供了新的靶点。
Objective: To investigate the effect of miR-188-5p on the expression of matrix metalloproteinases (MMPs) during the process of renal interstitial fibrosis. Methods: TGF-β1 was used to induce fibrosis of mesangial cells (MC), and the expression of miR-188-5p and MMP-13 was observed during the fibrosis process. The miR-188-5p On the regulatory role of MMP-13; liposome transfection method will be synthesized synthetic miR-188-5p mimics / inhibitor transfected into cells increase or decrease the expression of miR-188-5p further observation of miR-188-5p On the expression of MMP-13. Results: The expression of miR-188-5p was significantly increased and the expression of MMP-13 was down-regulated after TGF-β1-induced mesangial cell fibrosis. The reporter gene assay showed that miR-188-5p had a significant effect on the expression of MMP-13 The expression of miR-188-5p decreased the expression of MMP-13, while the expression of miR-188-5p decreased after the expression of MMP-13 was significantly negative regulation. Conclusion: In the process of renal interstitial fibrosis, miR-188-5p may play a role in promoting fibrosis by inhibiting the expression of MMP-13. This study provides a new target for the treatment of renal interstitial fibrosis.