论文部分内容阅读
A new fluorescent method was developed based on the ulifloxacin-europium(Ⅲ)-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium(Ⅲ)and significantly enhanced the characteristic fluorescence of europium(Ⅲ).The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0×10-8-2.0×10-6M with a detection limit of 2.0×10-10 M(3σ).This method is rapid and sensitive,and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.
A new fluorescent method was developed based on the ulifloxacin-europium (III) -sodium dodecylbenzene sulfonate system for the determination of ulifloxacin, the active metabolite of prulifloxacin. Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium (III) and significantly enhanced the characteristic fluorescence of europium (III). The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0 × 10 -8 -2.0 × 10 -6 M with a detection limit of 2.0 × 10 -10 M This method is rapid and sensitive, and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.