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目的分析胃癌血清及胃癌细胞培养液可见区激光拉曼光谱谱线特点,分析其量效特征,探讨特异性拉曼峰的来源。方法利用激光拉曼光谱仪对25份胃癌血清,10份功能性消化不良(FD)患者血清;不同时间点成组对照的 SGC7901胃癌细胞培养液及裂解液进行检测。结果以230为最小峰高计算机自动判读,在583,633,656,674,707,773和799 nm 7个波长上胃癌患者均可以检测到重复性好的拉曼峰,其中在633,656,674 nm 3波长处仅胃癌患者出现重复性较好的拉曼峰,而 FD 患者血清则未检测到可识别拉曼峰。在余下4个波长上胃癌血清荧光强度(96.5±6.0,76.9±4.7,63.7±4.5,285.7±18.6)均明显高于 FD 对照组(40.3±1.8,27.9±1.9,22.9±1.4,113.2±7.7,均 P<0.01);以100为最小峰高判读,在633,656,674 nm 3处胃癌细胞培养液可以检测出拉曼峰,而在1640培养基则不能检出;在583,707,773和799 nm 4处胃癌细胞培养液拉曼峰高显著高于1640培养基,且胃癌细胞培养液在上述7个波长拉曼峰高与培养时间呈正相关(Pearson 相关系数分别为0.845,0.875,0.875,0.873,0.805,0.891,0.880,均 P<0.01);胃癌细胞裂解液和培养液拉曼光谱在583,633,656 nm 3处两者的拉曼峰值差异均有统计学意义(11.1±3.2,5.0±0.9,3.3±0.5,均 P<0.05)。结论胃癌患者血清具有特异的,重复性较好的激光拉曼光谱谱像。激光拉曼光谱技术可以检测到不同浓度胃癌细胞代谢产物及裂解产物存在的差异。胃癌细胞凋亡后所释放的细胞内容物如 DNA、RNA 物质可能直接参与胃癌血清拉曼光谱的形成。
Objective To analyze the characteristics of laser Raman spectroscopy in the visible region of gastric cancer and gastric cancer cell culture medium and analyze its quantitative and efficacy characteristics and explore the source of specific Raman peak. Methods The serum of 25 gastric cancer patients and 10 patients with functional dyspepsia (FD) were analyzed by laser Raman spectroscopy. The SGC7901 gastric cancer cell culture medium and lysate were detected at different time points. The results were automatically interpreted by the computer with the minimum peak height of 230. The Raman peaks of the gastric cancer patients with the wavelength of 583, 633, 656, 674, 707, 773 and 799 nm could be detected with good reproducibility. Among them, only patients with gastric cancer at 633,656,674 nm 3 had better reproducibility Raman peak, while the FD patient serum did not detect recognizable Raman peak. The fluorescence intensity of gastric cancer at the remaining four wavelengths (96.5 ± 6.0,76.9 ± 4.7,63.7 ± 4.5,285.7 ± 18.6) were significantly higher than those in the FD control group (40.3 ± 1.8,27.9 ± 1.9,22.9 ± 1.4,113.2 ± 7.7 , All P <0.01). At the minimum peak height of 100, the Raman peaks were detected in the culture media of 633,656,674 nm 3 gastric cancer cells, but not in the 1640 medium. At 583,707,773 and 799 nm 4, The Raman peak height of the culture medium was significantly higher than that of the 1640 medium, and the Raman peak height of the gastric cancer cell culture medium at the above seven wavelengths was positively correlated with the culture time (Pearson correlation coefficients were 0.845,0.875,0.875,0.873,0.805,0.891, 0.880, all P <0.01). The Raman peak of gastric cancer cell lysate and culture solution at 583,633,656 nm 3 were statistically significant (11.1 ± 3.2,5.0 ± 0.9,3.3 ± 0.5, P <0.05). Conclusion The serum of patients with gastric cancer has specific and reproducible laser Raman spectroscopy. Laser Raman spectroscopy can detect differences in the metabolites and lysates of gastric cancer cells of different concentrations. The cellular contents such as DNA and RNA released after apoptosis of gastric cancer cells may be directly involved in the formation of gastric cancer Raman spectra.