Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana

• 作 者: 宋金柱 杨晓雪 王允 杨谦
• 机 构: Dept.ofLifeScienceandEngineering
• 出 处: 哈尔滨工业大学学报：英文版
• 发表日期: 2009年4期
• 关键词: 酿酒酵母 白僵菌 基因表达 克隆 几丁质酶基因 酵母表达载体 聚合酶链反应 质粒转化 Beauveria bassiana chitinase Sacch
• 基金项目: Sponsored by the Natural Science Foundation of Heilongjiang Province （ Grant No. C200609） and the National Science and Technology Supported Programe （ Grant No. 2006BAD07A01 ）.

To study recycled trashes from shrimps and crabs in the sea through chitinase secreted by microorganisms,the chitinase gene chit2 was cloned and sequenced from Beauveria bassiana by the polymerase chain reaction(PCR),and was ligated into the yeast express