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目的:监测西宁地区秋冬季婴幼儿轮状病毒感染情况,分析青海省主要流行株及其流行特点。方法:实验室采用巢式PCR方法对西宁地区婴幼儿腹泻的标本进行肠道病毒检测。结果:236份标本扩增后根据条带的大小进行分析,A组轮状病毒阳性25例,占总数的10.6%。其中G3型14例,占总数的5.9%,G9型11例,占总数的4.7%。结论:2010年西宁地区婴幼儿腹泻中轮状病毒是主要病原之一,发病多在6月龄～4岁的婴幼儿,男性患儿较女性患儿多,血清型主要为G3和G9型。
Objective: To monitor the status of rotavirus infection in infants and young children in Xining region in autumn and winter, and to analyze the prevalence of major epidemic strains in Qinghai Province. Methods: Nested PCR was used in laboratory to detect intestinal diarrhea in infants with diarrhea in Xining area. Results: According to the size of the bands after amplification, 236 samples were positive. A group of rotavirus positive 25 cases, accounting for 10.6% of the total. G3 type in which 14 cases, accounting for 5.9% of the total, G9 11 cases, accounting for 4.7% of the total. CONCLUSIONS: In 2010, rotavirus was one of the major pathogens in infant diarrhea in Xining area. Most of the infants were from 6 months old to 4 years old. There were more males than females with serogroup G3 and G9.