论文部分内容阅读
厌氧氨氧化(anaerobic ammonium oxidation,ANAMMOX)是潜流湿地净化污水的重要功能,目前关于潜流湿地厌氧氨氧化特征还不清楚.为了解处理水产养殖污水潜流湿地中的厌氧氨氧化特点,采用16S rRNA基因克隆文库和实时荧光定量PCR技术以及多样性分析等方法,对处理水产养殖污水潜流湿地中ANAMMOX菌的结构、多样性和丰度进行了研究分析.结果表明,处理水产养殖污水潜流湿地中存在Candidatus brocadia和Candidatus kuenenia这2类已知的ANAMMOX菌和3类未知的ANAMMOX菌,其优势种群为Candidatus brocadia;在不同季节中,秋季潜流湿地中ANAMMOX菌的多样性最高(H’,1.21),春季最低(H’,0.64);在养殖周期内,潜流湿地中ANAMMOX菌的丰度(以鲜重计,下同)在8.0×104~9.4×106copies·g-1之间,总细菌丰度在7.3×109~9.1×1010copies·g-1之间;在潜流湿地不同层面以及不同季节中,ANAMMOX菌的丰度存在着明显的差异,总细菌丰度则差异不明显,ANAMMOX菌的丰度在不同层面上的差异没有随时间变化的规律性.根据潜流湿地中ANAMMOX菌的分布特征,改变养殖污水布水方式和调整湿地结构可以提高潜流湿地的反硝化效果.该研究为进一步优化潜流湿地结构,提高净化养殖污水效率提供了依据.
Anaerobic ammonium oxidation (ANAMMOX) is an important function of sewage purification in sub-flow wetlands, and the characteristics of anaerobic ammonium oxidation in sub-flow wetlands are still unclear. In order to understand the characteristics of anaerobic ammonium oxidation in sub-flow wetland of aquaculture sewage, 16S rRNA gene cloning library, real-time fluorescence quantitative PCR and diversity analysis were used to analyze the structure, diversity and abundance of ANAMMOX bacteria in the aquaculture sewage undercurrent wetlands.The results showed that the treatment of the aquaculture sewage underflow wetlands Among the two known Candidaatus brocadia and Candidatus kuenenia, there are three known ANAMMOX and three unknown ANAMMOX bacteria, and the dominant species is Candidatus brocadia. In different seasons, the diversity of ANAMMOX bacteria in autumn underflow wetlands is the highest (H ’, 1.21 ), And the lowest in spring (H ’, 0.64). The abundance of ANAMMOX bacteria in subsurface wetlands (fresh weight, the same below) was between 8.0 × 104 ~ 9.4 × 106copies · g-1 during the breeding cycle. The abundance ranged from 7.3 × 109 to 9.1 × 1010copies · g-1. There was a significant difference in the abundance of ANAMMOX among different levels and under different seasons of underflow wetlands Obviously, the differences of ANAMMOX abundance at different levels did not change with time.According to the distribution characteristics of ANAMMOX bacteria in subsurface wetlands, changing the water distribution pattern of breeding sewage and adjusting wetland structure can improve the denitrification effect of underflow wetlands. The research provided the basis for further optimizing the underflow wetland structure and improving the efficiency of purifying aquaculture wastewater.