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应用地高辛（ｄｉｇｏｘｉｇｅｎｉｎ，ＤＩＧ）标记的生长抑素（Ｓｏｍａｔｏｓｔａｔｉｎ，ＳＯＭ）反义ＲＮＡ探针，对正常和胃癌组织进行原位杂交，研究ＳＯＭｍＲＮＡ的表达与胃癌发生、发展的关系。结果显示：在正常胃窦粘膜，杂交阳性细胞很少，散在分布于粘膜的中下部。胃癌组织中杂交阳性细胞显著增多（Ｐ＜０．０５）。进展期（Ⅲ～Ⅳ期）胃癌杂交阳性细胞明显多于早期（Ⅰ、Ⅱ期）胃癌（Ｐ＜０．０５）。结果揭示：ＳＯＭｍＲＮＡ转录和表达的改变参与胃癌发生发展的内分泌调节，并可作为胃癌预后的检测指标。
The somatostatin (SOM) antisense RNA probe labeled with digoxigenin (DIG) was used to detect the expression of SOM mRNA in gastric cancer and its relationship with the occurrence and development of gastric cancer. The results show that: in the normal antral mucosa, few hybrid positive cells, scattered in the lower part of the mucosa. The number of hybridization positive cells in gastric cancer tissues increased significantly (P <0.05). The number of positive hybridization cells in advanced stage (Ⅲ ~ Ⅳ stage) was significantly higher than that in early stages (Ⅰ, Ⅱ stage) (P <0.05). The results revealed that changes in SOM mRNA transcription and expression are involved in the regulation of endocrine development of gastric cancer and can be used as a detection index of prognosis of gastric cancer.