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目的 探讨慢性乙型肝炎患者血清TGFβ1、TNFα的水平与肝纤维化程度的关系。并从细胞因子角度探讨肝纤维化的发病机理。方法 肝穿刺取得肝脏活体组织进行HE、网状纤维染色 ,了解纤维化程度。采用ELISA法分别测定 2 0例正常人、10 0例慢性乙型肝炎及 2 4例肝硬化患者血清中TGFβ1和TNFα的水平。结果 1)慢性乙型肝炎轻、中、重度及肝硬化患者血清TGFβ1、TNFα水平均不同程度高于对照组 ,并且随着肝损害程度的加重逐渐升高。 2 )血清中TGFβ1、TNFα水平与肝纤维化程度呈正相关关系。结论 1)血清中TGFβ1、TNFα水平随着肝纤维化程度加重而升高 ,与肝纤维化发生发展密切相关。 2 )对血清中TGFβ1、TNFα水平同时测定 ,可作为诊断肝纤维化的血清学指标。 3)寻找有效的方法拮抗TGFβ1、TNFα对抗纤维化治疗具有重要意义。
Objective To investigate the relationship between serum levels of TGFβ1 and TNFα and the degree of liver fibrosis in patients with chronic hepatitis B. And from the perspective of cytokines to explore the pathogenesis of liver fibrosis. Methods Liver biopsies were obtained from liver biopsies for HE and reticular fiber staining to understand the degree of fibrosis. The levels of TGFβ1 and TNFα in 20 normal individuals, 100 chronic hepatitis B patients and 24 liver cirrhosis patients were determined by ELISA. Results 1) The serum levels of TGFβ1 and TNFα in patients with mild, moderate, severe and cirrhosis of chronic hepatitis B were all higher than those in the control group, and gradually increased with the severity of liver damage. 2) Serum TGFβ1, TNFα levels and the degree of liver fibrosis was positively correlated. Conclusions1) The levels of TGFβ1 and TNFα in serum are increased with the degree of liver fibrosis, which is closely related to the occurrence and development of hepatic fibrosis. 2) Simultaneous determination of TGFβ1 and TNFα levels in serum can be used as a serological marker for diagnosis of liver fibrosis. 3) looking for effective ways to antagonize TGFβ1, TNFα anti-fibrosis treatment of great significance.