新颖的人工合成α型干扰素(共有序列干扰素)的生物学活性及分子特性

• 作 者: 姚忻
• 出 处: 国外医学(微生物学分册)
• 发表日期: 1998年01期
• 关键词: 生物学活性 共有序列 人工合成 大肠杆菌表达 分子特性 种属特异性 活性试验 体外抗病毒 基因亚型 保守序列

共有序列(censensus)干扰素(IFN-conl)是一种人工合成的Ⅰ型IFN。通过分析多种自然IFN-α非等位基因亚型的氨基酸序列,在每个相应位置取最常出现的保守序列,然后将人工合成的相应DNA克隆于大肠杆菌表达质粒,通过细菌发酵产生含166个氨基酸残基的新IFN-α分子。 IFN-Conl的生物学活性具有种属特异性,其宿主范围与IFN-α2b类似。在体外抗病毒活性试验中.灵长类细胞能被较好地保护,仅次于人类组织细胞,而对其他种属细胞则基本无保护作用。白细胞IFN的多克隆

The censensus interferon (IFN-conl) is a synthetic type I IFN. By analyzing the amino acid sequences of a variety of natural IFN-a non-allelic subtypes, the most frequently occurring conserved sequence is taken at each of the corresponding positions, and then the corresponding synthetic DNA is cloned into an E. coli expression plasmid to produce a A new IFN-α molecule of 166 amino acid residues. The biological activity of IFN-Conl is species-specific and its host range is similar to IFN-α2b. In in vitro antiviral activity assays, primate cells are well protected, second only to human tissue cells, and have no protective effect on other species of cells. Polyclonal leukocyte IFN