Prokaryotic Expression of IBV N Protein and Development of Indirect IBV N Protein-mediated ELISA

• 作 者: Li Wei-qun Wang Xin Zhong Ming
• 机 构: KeyLaboratoryforLaboratoryAnimalsandComparativeMedicineofHeilongjiangProvince,CollegeofPharmaceutica
• 出 处: 东北农业大学学报:英文版
• 发表日期: 2020年1期
• 关键词: INFECTIOUS BRONCHITIS virus N protein PROKARYOTIC expression indirect ELISA
• 基金项目: Supported by the National Natural Science Foundation of China(31172295,31272569)

Avian infectious bronchitis(IB)is an acute and highly contagious disease caused by infectious bronchitis virus(IBV).In the study,according to IBV gene sequences published in Gen Bank,specific primers were designed to clone N gene by RT-PCR,and this gene w