论文部分内容阅读
进一步纯化了前一工作中从广西省产金环蛇(Bungarus fasciatus)蛇毒分离的突触后毒素Ⅰ和Ⅱ。以超饱和剂量的毒素Ⅰ或Ⅱ先与从电鳐(Narcine maculata)电器官得到的乙酰胆碱受体(AChR)保温10min 或 lh,再加入~(125)Ⅰ-标记α-银环蛇毒素或~(125)Ⅰ-标记眼镜蛇毒素,继续保温10min 或 lh,由测定与 AChR 结合的放射性强度得知,如以未经毒素Ⅰ或Ⅱ预饱和的放射性强度为100%,则经与其一预饱和者的约为30%,即毒素Ⅰ或Ⅱ只竞争地阻遏了α-银环蛇毒素或眼镜蛇毒素与 AChR 结合能力的2/3左右。文中讨论了存在两种类型 AChR 的可能性。
Further purification of the postsynaptic toxin I and II isolated from the venom of Bungarus fasciatus in Guangxi Province in the previous work. The supernatant dose of toxin I or II was first incubated with acetylcholine receptor (AChR) obtained from electrical organs of Narcine maculata for 10 min or 1 h followed by 125 I-labeled bungarotoxin or ~ (125) Ⅰ-labeled cobotoxin, and incubated for 10 or lh, determined by measuring the radioactivity of AChR binding, such as without radioactive pre-saturation toxins Ⅰ or Ⅱ intensity of 100%, then with a pre-saturator Of the approximately 30%, ie, toxin I or II only competitively inhibits about /3 of the binding capacity of a-bungarotoxin or cobraxin to AChR. The article discusses the possibility of two types of AChRs.