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上转换荧光材料在很多领域都具有新颖的应用潜力,然而其较低的发光效率给定量检测带来了难度。文章针对上转换荧光材料发光效率较低的特点,利用角锥棱镜的光学特性,设计了一套新型的上转换荧光检测光路。该装置将传统单工荧光检测光路扩展为双工系统,利用同轴多芯光纤从角锥棱镜两端同时接收荧光,提高了检测系统对微弱荧光的采集效率。在本研究中,设计了一系列实验来检验双工荧光收集光路的可行性,量化了荧光检测系统的线性度,从而证明了基于角锥棱镜上转换荧光检测系统的可行性。
Upconverting fluorescent materials have novel potential applications in many fields, however, their lower luminescent efficiency makes quantitative detection more difficult. Aiming at the low luminous efficiency of the upconverting fluorescent material, a novel set of upconverting fluorescence detecting optical path is designed by using the optical properties of the pyramidal prism. The device expands the traditional simplex fluorescence detection optical path to a duplex system, and utilizes the coaxial multi-core optical fiber to receive fluorescence simultaneously from both ends of the pyramid prism, thus improving the collection efficiency of the detection system to the weak fluorescence. In this study, we designed a series of experiments to test the feasibility of the duplex optical collection optical path, quantified the linearity of the fluorescence detection system, and proved the feasibility of the conversion fluorescence detection system based on the pyramid prism.