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为寻找新的疟疾病因性预防药物,我们建立了约氏鼠疟原虫—斯氏按蚊系统动物模型。实验证明,在一定条件下,斯氏按蚊的感染率为33±4.05％;小鼠易感性强,对照组的感染率相当稳定,可达94±0.011％。根据多次实验结果,提出了每鼠以一只阳性蚊感染,即时给药,连给三夭,然后在第7天和14天分别血检有无疟原虫,如阴性,则输血转种健康小鼠和切脾,再进行血检考核有无药效的简便过筛方法。在这一动物模型上,常用的抗疟药,如乙胺嘧啶、伯喹显示有病因性预防作用,而氯喹则无此作用。我们已用这一模型筛选了500余种化合物,证明方法可靠。至于如何区分药物对疟原虫红前期和红细胞内期的残留作用,在一般情况下区分并不十分困难,但对某些长效化合物,如有长效作用的喹哌等,则需通过食蟹猴疟原虫复筛,才能判定。
In search of a new malaria etiological prophylaxis, we established an animal model of S.japonicus-An. Experiments show that under certain conditions, An. Sinensis infection rate was 33 ± 4.05%; mice susceptible to strong, the control group infection rate was quite stable, up to 94 ± 0.011%. According to the results of multiple experiments, it is proposed that one positive mosquito be infected with each mouse, administered instantaneously, and given to three-day old mice. Blood samples were then tested for parasitemia on day 7 and 14, respectively. Mice and splenectomy, and then blood tests to check whether the efficacy of simple screening method. In this animal model, commonly used anti-malarial drugs such as pyrimethamine and primaquine showed etiological prophylaxis, whereas chloroquine did not. We have screened more than 500 compounds with this model, proving that the method is reliable. As for how to distinguish between drug residues on malaria prophase and erythrocytic period, it is not very difficult to distinguish in general, but for certain long-acting compounds, such as long-acting quinapopine, Plasmodium malaria re-screening, to determine.