Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmania

• 作 者: Joshua Muli Mutiso John Chege Macharia Evans Taracha Michael Muita Gicheru
• 机 构: Department of Tropical and Infectious Diseases,Institute of Primate Research,Department of Zoologica
• 出 处: Journal of Biomedical Research
• 发表日期: 2012年01期
• 关键词: visceral delivered vaccine monkey vaccines adjuvant vaccination interferon prote

In a previous immunogenicity and efficacy study in mice,montanide ISA 720(MISA)was indicated to be a better adjuvant than bacillus calmette guerin vaccine(BCG)for a Leishmania vaccine.In the present study,we report the safety,immunogenicity and efficacy of Leishmania donovani(L.donovani)sonicated antigen delivered with alum-BCG(AlBCG),MISA or monophosphoryl lipid A(MPLA)in vervet monkeys following intradermal inoculums.Vaccinated and control animals were challenged with virulent L.donovani parasites and the parasitic burden was determined.Only animals vaccinated with alum-BCG adversely reacted to the inoculum by producing ulcerative erythematous skin indurations.Non-parametric ANOVA followed by a post test showed significantly higher IgG antibodies,and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups(P < 0.001).We conclude that L.donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.

In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined.Only animals vaccinated with alum-BCG adversely reacted to the inoculum by producing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showing greater IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG + Ag and MISA + Ag as compared to the MPLA + Ag or other groups (P <0.001) .We conclude that L.donovani sonicated antigen containing MISA is safe and is a ssociated with protective immune response against Leishmania donovani infection in the vervet monkey model.