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为了探索在真核细胞内转录表达乙型肝炎病毒（ＨＢＶ）反义核酸的方法，用基因重组技术将ＨＢＶ前Ｃ／Ｃ基因（ＰｒｅＣ／Ｃ）和前Ｓ／Ｓ基因（ＰｒｅＳ／Ｓ）片段反向插入逆转录病毒载体质粒，再将重组体分别转染ＰＡ３１７包装细胞，进而获得能够介导ＨＢＶ反义基因向小鼠ＮＩＨ３Ｔ３细胞转移表达的重组逆转录病毒。经分子杂交试验表明，含有ＨＢＶ反义基因的重组逆转录病毒序列已经整合到转染的ＰＡ３１７细胞染色体上；转导的ＮＩＨ３Ｔ３细胞内有ＨＢＶ反义ＲＮＡ转录表达。结论：逆转录病毒载体包装细胞系统能够介导ＨＢＶ反义基因在真核细胞中转录表达，因而有可能利用反义技术和基因转移方法进行抗－ＨＢＶ基因治疗
In order to explore a method of transcribing and expressing hepatitis B virus (HBV) antisense nucleic acid in eukaryotic cells, the preC / C gene and preS / S gene (PreS / S) Reversely inserted into the retroviral vector plasmid, and then the recombinant were transfected PA317 packaging cells, and then obtain a recombinant retroviral vector that can mediate the transfer of HBV antisense gene to mouse NIH3T3 cells. The molecular hybridization test showed that the recombinant retroviral sequence containing HBV antisense gene has been integrated into the chromosome of transfected PA317 cells; transduced NIH3T3 cells have HBV antisense RNA transcripts. CONCLUSIONS: The retroviral vector packaging cell system can mediate the transcriptional expression of HBV antisense gene in eukaryotic cells, making it possible to use antisense technology and gene transfer methods for anti-HBV gene therapy