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目的:通过对肥胖哮喘动物模型发病情况、介质变化的研究,探讨肥胖在哮喘发病中的作用。方法:将雄性Wistar大鼠随机分为肥胖哮喘组(A组)、哮喘组(B组)和对照组(C组)3组各10只。A组以高脂饲料喂养,B、C组以普通饲料喂养。A、B组大鼠腹腔注射并两周后雾化吸入卵清蛋白(OVA)建立哮喘模型。取肺组织做病理切片,应用免疫组化方法检测肺组织肿瘤坏死因子-α(TNF-α)水平,ELISA双抗体夹心法测定血浆白细胞介素-8(IL-8)水平。结果:A组大鼠气道壁增厚、炎症细胞浸润均较其他两组明显,肺组织TNF-α水平(25%±3%)高于B组(16%±3%)及C组(9%±2%)(均P<0.05),血浆IL-8水平[(32.70±7.95)ng.L-1)]高于B组[(24.52±3.03)ng.L-1]及C组[(17.48±3.06)ng.L-1](均P<0.05)。结论:肥胖影响哮喘发作严重程度以及体内某些哮喘相关因子的含量。
OBJECTIVE: To explore the role of obesity in the pathogenesis of asthma through the study of the incidence and the change of media in obese asthma animal models. Methods: Male Wistar rats were randomly divided into three groups: obese asthma group (group A), asthma group (group B) and control group (group C). Group A was fed with high-fat diet, Group B and C were fed with normal diet. Rats in group A and group B were injected intraperitoneally with nebulized ovalbumin (OVA) two weeks later to establish an asthma model. The lung tissue was taken for pathological section. The level of tumor necrosis factor-α (TNF-α) in lung tissue was detected by immunohistochemistry and the level of plasma interleukin-8 (IL-8) by ELISA. Results: The thickening of airway wall and the infiltration of inflammatory cells in group A were more obvious than those in the other two groups. The level of TNF-α in lung tissue was higher (25% ± 3%) than that in group B (16% ± 3%) and group C The level of IL-8 in plasma [(32.70 ± 7.95) ng.L-1) was significantly higher than that in group B [(24.52 ± 3.03) ng.L-1 and 9% ± 2%, respectively [(17.48 ± 3.06) ng.L-1] (all P <0.05). Conclusion: Obesity affects the severity of asthma attacks and some asthma-related factors in the body.